Coding
Part:BBa_K2510016
Designed by: Alicia Graham Group: iGEM17_Paris_Bettencourt (2017-10-27)
PhaA and PhaB under T3 Promoter and PhaC under K1F Promoter
PhaA and PhaB under the T3 promoter and with a double terminator with PhaC . These are the three enzymes in PH3B production pathway. Separating them under two different promoters allows the creation of logic gates. This part lacks the terminator at the end, found in the design of BBa_K2510060.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 597
Illegal BglII site found at 1422 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 574
Illegal NgoMIV site found at 886
Illegal NgoMIV site found at 1165
Illegal NgoMIV site found at 2550
Illegal NgoMIV site found at 2691
Illegal NgoMIV site found at 2858
Illegal NgoMIV site found at 3704 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3772
[edit]
Categories
Parameters
None |